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KMID : 1005220110260030219
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Journal of Embryo Transfer
2011 Volume.26 No. 3 p.219 ~ p.222
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Changes of Sex Hormones and Cervical Mucus in Estrus-synchronized Himalayan Tahrs (Hemitragus jemlahicus) in Non-breeding Season
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Yong Hwan-Yul
Kim Myoung-Ho
Ha Yong-Hee
Kim Han-Woong
Kim Mi-Young
Cho Dong-Gil
Oh Chang-Sik
Kim Doo-Hee
Lim Yang-Mook
Yoo Mi-Hyun
Bae Bok-Soo
Kim Seung-Dong
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Abstract
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Eight female Himalayan tahrs (Hemitragus jemlahicus) were estrus-synchronized, and transcervically inseminated with frozen-thawed semen in September, 2009, about 2 to 3 months earlier than their natural breeding season. Intravaginal progesterone-releasing devices were inserted into vaginas of six Himalayan tahrs on September 7, and the other two on September 8 to suppress luteal function of ovaries. The devices had been placed deep inside the vagina prior to withdrawal on September 23. A day before CIDR removal, a combination of PMSG 400 IU and hCG 200 IU was intramuscularly injected. Forty hours later, frozen-thawed semen was transcervically inseminated. Pregnancy diagnosis was performed 39 days later by analyzing progesterone level of serum. Every treatment was done under anesthesia inducted by xylazine injection. In conclusion, vaginal discharge of cervical mucus, hormonal changes induced by implant-typed or muscularly injectable hormones and widening of cervix enough to insert an insemination gun into uterine body were achieved in non-breeding season. Moreover, the first inseminated Himalayan tahr, 36 hours after CIDR removal was assumed to be pregnant but the fetus may have been lost due to the use of anesthetic drug.
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KEYWORD
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Himalayan tahr , estrus synchronization , semen , cryopreservation , laparoscopic , insemination
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